A high-throughput alphavirus-based expression cloning system for mammalian cells.

We have developed a widely applicable functional genomics strategy based on alphavirus expression vectors. The technology allows for rapid identification of genes encoding a functional activity such as binding of a defined ligand. Complementary DNA (cDNA) libraries were expressed in mammalian cells...

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Bibliographic Details
Main Authors: Koller, D, Ruedl, C, Loetscher, M, Vlach, J, Oehen, S, Oertle, K, Schirinzi, M, Deneuve, E, Moser, R, Kopf, M, Bailey, J, Renner, W, Bachmann, M
Format: Journal article
Language:English
Published: 2001