A high-throughput alphavirus-based expression cloning system for mammalian cells.
We have developed a widely applicable functional genomics strategy based on alphavirus expression vectors. The technology allows for rapid identification of genes encoding a functional activity such as binding of a defined ligand. Complementary DNA (cDNA) libraries were expressed in mammalian cells...
Main Authors: | , , , , , , , , , , , , |
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Format: | Journal article |
Language: | English |
Published: |
2001
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