Robust quantification of live-cell single-molecule tracking data for fluorophores with different photophysical properties
High-speed single-molecule tracking in live cells is becoming an increasingly popular method for quantifying the spatiotemporal behavior of proteins in vivo. The method provides a wealth of quantitative information, but users need to be aware of biases that can skew estimates of molecular mobilities...
Main Authors: | , |
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Format: | Journal article |
Language: | English |
Published: |
American Chemical Society
2024
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