Robust quantification of live-cell single-molecule tracking data for fluorophores with different photophysical properties

Robust quantification of live-cell single-molecule tracking data for fluorophores with different photophysical properties

High-speed single-molecule tracking in live cells is becoming an increasingly popular method for quantifying the spatiotemporal behavior of proteins in vivo. The method provides a wealth of quantitative information, but users need to be aware of biases that can skew estimates of molecular mobilities...

书目详细资料
Main Authors:	Moores, AN, Uphoff, S
格式:	Journal article
语言:	English
出版:	American Chemical Society 2024

相似书籍

Single molecule imaging simulations with advanced fluorophore photophysics
由: Dominique Bourgeois
出版: (2023-01-01)

Tailor-Made Dynamic Fluorophores: Precise Structures Controlling the Photophysical Properties
由: Shuhai Qiu, et al.
出版: (2023-02-01)

Research Progress of Fluorescent Probes for Detection of Glutathione (GSH): Fluorophore, Photophysical Properties, Biological Applications
由: Yao Wang, et al.
出版: (2024-09-01)

Visualizing protein-DNA interactions in live bacterial cells using photoactivated single-molecule tracking.
由: Uphoff, S, et al.
出版: (2014)

Graphitic Dots Combining Photophysical Characteristics of Organic Molecular Fluorophores and Inorganic Quantum Dots
由: Raphaël de Boëver, et al.
出版: (2021-05-01)

Photophysics of aromatic molecules /
由: 280690 Birks, John B.
出版: (1970)

Photophysics of molecular materials : from single molecules to single crystals /
由: Lanzani, Guglielmo
出版: (2006)

Understanding protein mobility in bacteria by tracking single molecules
由: Kapanidis, A, et al.
出版: (2018)

Switchable and Functional Fluorophores for Multidimensional Single-Molecule Localization Microscopy
由: Yunshu Liu, et al.
出版: (2023-06-01)

Quantification of Small Molecule–Protein Interactions using FRET between Tryptophan and the Pacific Blue Fluorophore
由: Molly M. Lee, et al.
出版: (2016-12-01)

Synthesis and Photophysical Properties of α-(<i>N</i>-Biphenyl)-Substituted 2,2′-Bipyridine-Based Push–Pull Fluorophores
由: Ekaterina S. Starnovskaya, et al.
出版: (2022-10-01)

Theoretical and experimental studies on electronic structure and photophysical properties of biological active substituted hydroxyquinolin-2(1H)-one fluorophore
由: N. Sunil Kumar, et al.
出版: (2023-06-01)

Choosing the right label for single-molecule tracking in live bacteria: side-by-side comparison of photoactivatable fluorescent protein and Halo tag dyes
由: Banaz, N, et al.
出版: (2018)

Correlative Extinction and Single Fluorophore Bleaching Microscopy for Ligand Quantification on Gold Nanoparticles
由: Nicole Slesiona, et al.
出版: (2023-12-01)

Robust nonparametric quantification of clustering density of molecules in single-molecule localization microscopy.
由: Shenghang Jiang, et al.
出版: (2017-01-01)

Single-molecule DNA repair in live bacteria.
由: Uphoff, S, et al.
出版: (2013)

Long-lived intracellular single-molecule fluorescence using electroporated molecules.
由: Crawford, R, et al.
出版: (2013)

Absolute measurement of cellular activities using photochromic single-fluorophore biosensors and intermittent quantification
由: Franziska Bierbuesse, et al.
出版: (2022-04-01)

Redox-Modulations of Photophysical and Single-molecule Magnet Properties in Ytterbium Complexes Involving Extended-TTF Triads
由: Bertrand Lefeuvre, et al.
出版: (2020-01-01)

Tracking single particles for hours via continuous DNA-mediated fluorophore exchange
由: Florian Stehr, et al.
出版: (2021-07-01)

Photophysical properties of electroluminescent molecules based on thiophene and oxadiazole. Computational investigations
由: Fatima Agda, et al.
出版: (2020-01-01)

Internalization of Fluorescent Biomolecules for Long-Lived Single-Molecule Observation in Living Bacteria
由: Crawford, R, et al.
出版: (2013)

A Multi-Fluorophore Staining Scheme for Identification and Quantification of Vomocytosis
由: Noah Pacifici, et al.
出版: (2023-08-01)

Synthesis of a 5-Carboxy Indole-Based Spiropyran Fluorophore: Thermal, Electrochemical, Photophysical and Bovine Serum Albumin Interaction Investigations
由: Rodrigo da Costa Duarte, et al.
出版: (2020-05-01)

Fluorophore Labeling, Nanodisc Reconstitution and Single-molecule Observation of a G Protein-coupled Receptor
由: Rajan Lamichhane, et al.
出版: (2017-06-01)

Tethered fluorophore motion: studying large DNA conformational changes by single-fluorophore imaging.
由: May, P, et al.
出版: (2014)

Author Correction: Absolute measurement of cellular activities using photochromic single-fluorophore biosensors and intermittent quantification
由: Franziska Bierbuesse, et al.
出版: (2023-04-01)

Colour-coded nanoscale calibration and optical quantification of axial fluorophore position
由: Olevsko-Arad Ilya, et al.
出版: (2023-01-01)

A single-molecule method for measuring fluorophore labeling yields for the study of membrane protein oligomerization in membranes.
由: Melanie Ernst, et al.
出版: (2023-01-01)

A Toolbox for Site-Specific Labeling of RecQ Helicase With a Single Fluorophore Used in the Single-Molecule Assay
由: Fang-Yuan Teng, et al.
出版: (2020-09-01)

Light sheet microscopy for single molecule tracking in living tissue.
由: Jörg Gerhard Ritter, et al.
出版: (2010-01-01)

A study of photophysical processes using raman and fluorescence spectroscopy at the ensemble and single molecule levels
由: Jefri Sanusi Teguh
出版: (2014)

Single-molecule trapping and spectroscopy reveals photophysical heterogeneity of phycobilisomes quenched by Orange Carotenoid Protein
由: Allison H. Squires, et al.
出版: (2019-03-01)

Studying the organization of DNA repair by single-cell and single-molecule imaging.
由: Uphoff, S, et al.
出版: (2014)

Tracking tumor heterogeneity and progression with near‐infrared II fluorophores
由: Qi Xin, et al.
出版: (2023-04-01)

Characterization of organic fluorophores for in vivo FRET studies based on electroporated molecules.
由: Plochowietz, A, et al.
出版: (2014)

Single-Molecule Tracking in Living Cells Using Single Quantum Dot Applications
由: Koichi Baba, Kohji Nishida
出版: (2012-01-01)

Multicolor fluorescence nanoscopy in fixed and living cells by exciting conventional fluorophores with a single wavelength.
由: Testa, I, et al.
出版: (2010)

Single-molecule analysis of bacterial DNA repair and mutagenesis
由: Uphoff, S, et al.
出版: (2017)

Photophysical Properties of Chlorotriethylphosphinegold(I)
由: Kang, Jun-Gill, et al.
出版: (2010)