Novel homogenous time-resolved fluorometric RT-PCR assays for quantification of PSA and hK2 mRNAs in blood.

OBJECTIVES: The purpose of this study was to design, validate, and optimize internally standardized real-time quantitative RT-PCR assays and to identify and avoid problems with assay reliability and examine the impact of an exogenous internal standard. DESIGN AND METHODS: The model system consisted...

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Bibliographic Details
Main Authors: Rissanen, M, Helo, P, Väänänen, R, Wahlroos, V, Lilja, H, Nurmi, M, Pettersson, K, Nurmi, J
Format: Journal article
Language:English
Published: 2007