Ultra acidic strong cation exchange enabling the efficient enrichment of basic phosphopeptides.
We present a straightforward method to enrich phosphopeptides with multiple basic residues, an under-represented class in common enrichment strategies. Our method is based on a two-dimensional strong cation exchange (SCX) strategy, operating at two different acidic pHs, enabling both separation and...
| Hlavní autoři: | , , , , |
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| Médium: | Journal article |
| Jazyk: | English |
| Vydáno: |
2012
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| _version_ | 1826290549569617920 |
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| author | Hennrich, M van den Toorn, H Groenewold, V Heck, A Mohammed, S |
| author_facet | Hennrich, M van den Toorn, H Groenewold, V Heck, A Mohammed, S |
| author_sort | Hennrich, M |
| collection | OXFORD |
| description | We present a straightforward method to enrich phosphopeptides with multiple basic residues, an under-represented class in common enrichment strategies. Our method is based on a two-dimensional strong cation exchange (SCX) strategy, operating at two different acidic pHs, enabling both separation and enrichment of different classes of phosphopeptides. The principle of enrichment is based on the change of net charge of phosphorylated peptides under strong acidic conditions in the second SCX, whereas the net charge of regular peptides remains unchanged, thus enabling separation based on net charge. Application of our tandem SCX approach to a modest amount of human cells allowed the identification of over 10,000 unique "basic" phosphopeptides of which many represent putative targets of basophilic kinases. |
| first_indexed | 2024-03-07T02:45:53Z |
| format | Journal article |
| id | oxford-uuid:ac0ec007-2a6f-455a-b4e5-45de77274162 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:45:53Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | oxford-uuid:ac0ec007-2a6f-455a-b4e5-45de772741622022-03-27T03:26:03ZUltra acidic strong cation exchange enabling the efficient enrichment of basic phosphopeptides.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ac0ec007-2a6f-455a-b4e5-45de77274162EnglishSymplectic Elements at Oxford2012Hennrich, Mvan den Toorn, HGroenewold, VHeck, AMohammed, SWe present a straightforward method to enrich phosphopeptides with multiple basic residues, an under-represented class in common enrichment strategies. Our method is based on a two-dimensional strong cation exchange (SCX) strategy, operating at two different acidic pHs, enabling both separation and enrichment of different classes of phosphopeptides. The principle of enrichment is based on the change of net charge of phosphorylated peptides under strong acidic conditions in the second SCX, whereas the net charge of regular peptides remains unchanged, thus enabling separation based on net charge. Application of our tandem SCX approach to a modest amount of human cells allowed the identification of over 10,000 unique "basic" phosphopeptides of which many represent putative targets of basophilic kinases. |
| spellingShingle | Hennrich, M van den Toorn, H Groenewold, V Heck, A Mohammed, S Ultra acidic strong cation exchange enabling the efficient enrichment of basic phosphopeptides. |
| title | Ultra acidic strong cation exchange enabling the efficient enrichment of basic phosphopeptides. |
| title_full | Ultra acidic strong cation exchange enabling the efficient enrichment of basic phosphopeptides. |
| title_fullStr | Ultra acidic strong cation exchange enabling the efficient enrichment of basic phosphopeptides. |
| title_full_unstemmed | Ultra acidic strong cation exchange enabling the efficient enrichment of basic phosphopeptides. |
| title_short | Ultra acidic strong cation exchange enabling the efficient enrichment of basic phosphopeptides. |
| title_sort | ultra acidic strong cation exchange enabling the efficient enrichment of basic phosphopeptides |
| work_keys_str_mv | AT hennrichm ultraacidicstrongcationexchangeenablingtheefficientenrichmentofbasicphosphopeptides AT vandentoornh ultraacidicstrongcationexchangeenablingtheefficientenrichmentofbasicphosphopeptides AT groenewoldv ultraacidicstrongcationexchangeenablingtheefficientenrichmentofbasicphosphopeptides AT hecka ultraacidicstrongcationexchangeenablingtheefficientenrichmentofbasicphosphopeptides AT mohammeds ultraacidicstrongcationexchangeenablingtheefficientenrichmentofbasicphosphopeptides |