Importance of antigen specificity for complement-mediated lysis by monoclonal antibodies.
Lysis of human lymphocytes by autologous complement had been studied using a range of monoclonal antibodies against different antigens. Antigen specificity (and not antibody isotype) was the most important factor which influenced cell lysis and this could not be accounted for merely by differences i...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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1988
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| _version_ | 1826290565285675008 |
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| author | Bindon, C Hale, G Waldmann, H |
| author_facet | Bindon, C Hale, G Waldmann, H |
| author_sort | Bindon, C |
| collection | OXFORD |
| description | Lysis of human lymphocytes by autologous complement had been studied using a range of monoclonal antibodies against different antigens. Antigen specificity (and not antibody isotype) was the most important factor which influenced cell lysis and this could not be accounted for merely by differences in surface density between antigens. Three antigens with comparable surface density were studied in detail: CAMPATH-1 (lytic), major histocompatibility complex class I (lytic) and leukocyte common antigen (poorly lytic). C1q binding was roughly proportional to antibody binding and dependent on antibody isotype. However, the lytic antibodies were much better able to bind and activate whole C1 than the poorly lytic ones. This result would not have been predicted from traditional concepts of complement activation but can be interpreted in the light of models for C1 activation which involve Fc-Fc interactions, Fc-C1r2s2 interactions and a critical C1q stem-arm angle for C1 binding and activation. |
| first_indexed | 2024-03-07T02:46:08Z |
| format | Journal article |
| id | oxford-uuid:ac244151-d478-4336-89ed-678bab1eda87 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:46:08Z |
| publishDate | 1988 |
| record_format | dspace |
| spelling | oxford-uuid:ac244151-d478-4336-89ed-678bab1eda872022-03-27T03:26:31ZImportance of antigen specificity for complement-mediated lysis by monoclonal antibodies.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ac244151-d478-4336-89ed-678bab1eda87EnglishSymplectic Elements at Oxford1988Bindon, CHale, GWaldmann, HLysis of human lymphocytes by autologous complement had been studied using a range of monoclonal antibodies against different antigens. Antigen specificity (and not antibody isotype) was the most important factor which influenced cell lysis and this could not be accounted for merely by differences in surface density between antigens. Three antigens with comparable surface density were studied in detail: CAMPATH-1 (lytic), major histocompatibility complex class I (lytic) and leukocyte common antigen (poorly lytic). C1q binding was roughly proportional to antibody binding and dependent on antibody isotype. However, the lytic antibodies were much better able to bind and activate whole C1 than the poorly lytic ones. This result would not have been predicted from traditional concepts of complement activation but can be interpreted in the light of models for C1 activation which involve Fc-Fc interactions, Fc-C1r2s2 interactions and a critical C1q stem-arm angle for C1 binding and activation. |
| spellingShingle | Bindon, C Hale, G Waldmann, H Importance of antigen specificity for complement-mediated lysis by monoclonal antibodies. |
| title | Importance of antigen specificity for complement-mediated lysis by monoclonal antibodies. |
| title_full | Importance of antigen specificity for complement-mediated lysis by monoclonal antibodies. |
| title_fullStr | Importance of antigen specificity for complement-mediated lysis by monoclonal antibodies. |
| title_full_unstemmed | Importance of antigen specificity for complement-mediated lysis by monoclonal antibodies. |
| title_short | Importance of antigen specificity for complement-mediated lysis by monoclonal antibodies. |
| title_sort | importance of antigen specificity for complement mediated lysis by monoclonal antibodies |
| work_keys_str_mv | AT bindonc importanceofantigenspecificityforcomplementmediatedlysisbymonoclonalantibodies AT haleg importanceofantigenspecificityforcomplementmediatedlysisbymonoclonalantibodies AT waldmannh importanceofantigenspecificityforcomplementmediatedlysisbymonoclonalantibodies |