Characterisation of the dominant oxidative folding intermediate of hen lysozyme.
Reduced denatured lysozyme has been oxidised and refolded at pH values close to neutral in an efficient way by dilution from buffers containing 8.0 M urea, and refolding intermediates were separated by reverse-phase HPLC at pH 2. By using peptic digestion in combination with high-resolution Fourier...
| Główni autorzy: | , , , |
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| Format: | Journal article |
| Język: | English |
| Wydane: |
1999
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