QuaNCAT: Quantitating proteome dynamics in primary cells

Here we demonstrate quantitation of stimuli-induced proteome dynamics in primary cells by combining the power of bio-orthogonal noncanonical amino acid tagging (BONCAT) and stable-isotope labeling of amino acids in cell culture (SILAC). In conjunction with nanoscale liquid chromatography-tandem mass...

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Bibliographic Details
Main Authors: Howden, A, Geoghegan, V, Katsch, K, Efstathiou, G, Bhushan, B, Boutureira, O, Thomas, B, Trudgian, D, Kessler, B, Dieterich, D, Davis, BG, Acuto, O
Format: Journal article
Language:English
Published: 2013