Effect of cryopreservation of peripheral blood mononuclear cells (PBMCs) on the variability of an antigen-specific memory B cell ELISpot.

The ELISpot assay is used in vaccine studies for the quantification of antigen-specific memory B cells (B(MEM)), and can be performed using cryopreserved samples. The effects of cryopreservation on B(MEM) detection and the consistency of cultured ELISpot assays when performed by different operators...

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Xehetasun bibliografikoak
Egile Nagusiak: Trück, J, Mitchell, R, Thompson, A, Morales-Aza, B, Clutterbuck, E, Kelly, D, Finn, A, Pollard, A
Formatua: Journal article
Hizkuntza:English
Argitaratua: 2014