Quantification of siRNA using competitive qPCR.
We have developed a PCR-based short interfering RNA (siRNA) quantification method based on competition between siRNA and a homologous DNA primer for annealing to template DNA, avoiding the requirement for prior conversion of RNA to cDNA. Primers and probe were designed to amplify regions of the huma...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Oxford University Press
2009
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