Quantification of siRNA using competitive qPCR.

Quantification of siRNA using competitive qPCR.

We have developed a PCR-based short interfering RNA (siRNA) quantification method based on competition between siRNA and a homologous DNA primer for annealing to template DNA, avoiding the requirement for prior conversion of RNA to cDNA. Primers and probe were designed to amplify regions of the huma...

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Dades bibliogràfiques
Autors principals:	Liu, W, Stevenson, M, Seymour, L, Fisher, K
Format:	Journal article
Idioma:	English
Publicat:	Oxford University Press 2009

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