Quantification of siRNA using competitive qPCR.
We have developed a PCR-based short interfering RNA (siRNA) quantification method based on competition between siRNA and a homologous DNA primer for annealing to template DNA, avoiding the requirement for prior conversion of RNA to cDNA. Primers and probe were designed to amplify regions of the huma...
| Auteurs principaux: | , , , |
|---|---|
| Format: | Journal article |
| Langue: | English |
| Publié: |
Oxford University Press
2009
|