Quantification of siRNA using competitive qPCR.

Quantification of siRNA using competitive qPCR.

We have developed a PCR-based short interfering RNA (siRNA) quantification method based on competition between siRNA and a homologous DNA primer for annealing to template DNA, avoiding the requirement for prior conversion of RNA to cDNA. Primers and probe were designed to amplify regions of the huma...

書誌詳細
主要な著者:	Liu, W, Stevenson, M, Seymour, L, Fisher, K
フォーマット:	Journal article
言語:	English
出版事項:	Oxford University Press 2009

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