Quantification of siRNA using competitive qPCR.

Quantification of siRNA using competitive qPCR.

We have developed a PCR-based short interfering RNA (siRNA) quantification method based on competition between siRNA and a homologous DNA primer for annealing to template DNA, avoiding the requirement for prior conversion of RNA to cDNA. Primers and probe were designed to amplify regions of the huma...

书目详细资料
Main Authors:	Liu, W, Stevenson, M, Seymour, L, Fisher, K
格式:	Journal article
语言:	English
出版:	Oxford University Press 2009

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