Pseudotyped influenza A virus as a vaccine for the induction of heterotypic immunity.
There is a need for vaccines that can protect broadly across all influenza A strains. We have produced a pseudotyped influenza virus based on suppression of the A/PR/8/34 hemagglutinin signal sequence (S-FLU) that can infect cells and express the viral core proteins and neuraminidase but cannot repl...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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2012
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| _version_ | 1797089791524732928 |
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| author | Powell, T Silk, J Sharps, J Fodor, E Townsend, A |
| author_facet | Powell, T Silk, J Sharps, J Fodor, E Townsend, A |
| author_sort | Powell, T |
| collection | OXFORD |
| description | There is a need for vaccines that can protect broadly across all influenza A strains. We have produced a pseudotyped influenza virus based on suppression of the A/PR/8/34 hemagglutinin signal sequence (S-FLU) that can infect cells and express the viral core proteins and neuraminidase but cannot replicate. We show that when given by inhalation to mice, S-FLU is nonpathogenic but generates a vigorous T cell response in the lung associated with markedly reduced viral titers and weight loss after challenge with H1 and H3 influenza viruses. These properties of S-FLU suggest that it may have potential as a broadly protective A virus vaccine, particularly in the setting of a threatened pandemic before matched subunit vaccines become available. |
| first_indexed | 2024-03-07T03:09:10Z |
| format | Journal article |
| id | oxford-uuid:b397639f-62af-4955-a3fd-df27e3fe368d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:09:10Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | oxford-uuid:b397639f-62af-4955-a3fd-df27e3fe368d2022-03-27T04:20:18ZPseudotyped influenza A virus as a vaccine for the induction of heterotypic immunity.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:b397639f-62af-4955-a3fd-df27e3fe368dEnglishSymplectic Elements at Oxford2012Powell, TSilk, JSharps, JFodor, ETownsend, AThere is a need for vaccines that can protect broadly across all influenza A strains. We have produced a pseudotyped influenza virus based on suppression of the A/PR/8/34 hemagglutinin signal sequence (S-FLU) that can infect cells and express the viral core proteins and neuraminidase but cannot replicate. We show that when given by inhalation to mice, S-FLU is nonpathogenic but generates a vigorous T cell response in the lung associated with markedly reduced viral titers and weight loss after challenge with H1 and H3 influenza viruses. These properties of S-FLU suggest that it may have potential as a broadly protective A virus vaccine, particularly in the setting of a threatened pandemic before matched subunit vaccines become available. |
| spellingShingle | Powell, T Silk, J Sharps, J Fodor, E Townsend, A Pseudotyped influenza A virus as a vaccine for the induction of heterotypic immunity. |
| title | Pseudotyped influenza A virus as a vaccine for the induction of heterotypic immunity. |
| title_full | Pseudotyped influenza A virus as a vaccine for the induction of heterotypic immunity. |
| title_fullStr | Pseudotyped influenza A virus as a vaccine for the induction of heterotypic immunity. |
| title_full_unstemmed | Pseudotyped influenza A virus as a vaccine for the induction of heterotypic immunity. |
| title_short | Pseudotyped influenza A virus as a vaccine for the induction of heterotypic immunity. |
| title_sort | pseudotyped influenza a virus as a vaccine for the induction of heterotypic immunity |
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