Covalent attachment of an electroactive sulfydryl reagent in the active site of cytochrome P450(cam) as revealed by the crystal structure of the modified protein
A novel electroactive sulfydryl-specific reagent, N-(2-ferrocenylethyl)maleimide (Fc-Mi), was used to attach a redox-active reporter group to cytochrome P450(cam) from Pseudomonas putida. The crystal structure of the modified enzyme was determined at 2.2 Å resolution (R(cryst) = 0.18) and compared t...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1998
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