Multicolor fluorescence nanoscopy in fixed and living cells by exciting conventional fluorophores with a single wavelength.

Current far-field fluorescence nanoscopes provide subdiffraction resolution by exploiting a mechanism of fluorescence inhibition. This mechanism is implemented such that features closer than the diffraction limit emit separately when simultaneously exposed to excitation light. A basic mechanism for...

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Bibliographic Details
Main Authors: Testa, I, Wurm, C, Medda, R, Rothermel, E, von Middendorf, C, Fölling, J, Jakobs, S, Schönle, A, Hell, S, Eggeling, C
Format: Journal article
Language:English
Published: 2010