Triplet-relaxation microscopy with bunched pulsed excitation.

Obtaining high signal levels in fluorescence microscopy is usually spoiled by the concomitant population of the dark (triplet) state of the marker, which is often followed by photobleaching. Recently, we introduced the triplet relaxation (T-Rex) modality in fluorescence microscopy which led to a maj...

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Bibliographic Details
Main Authors: Donnert, G, Eggeling, C, Hell, S
Format: Journal article
Language:English
Published: 2009