Proteomic analysis of the cell cycle of procylic form Trypanosoma brucei

We describe a single-step centrifugal elutriation method to produce synchronous G1-phase procyclic trypanosomes at a scale amenable for proteomic analysis of the cell cycle. Using ten-plex tandem mass tag (TMT) labelling and mass spectrometry (MS)-based proteomics technology, the expression levels o...

Повний опис

Бібліографічні деталі
Автори: Crozier, TWM, Tinti, M, Wheeler, RJ, Ly, T, Ferguson, MAJ, Lamond, AI
Формат: Journal article
Мова:English
Опубліковано: American Society for Biochemistry and Molecular Biology 2018