Vitrification of corneal endothelial cells in a monolayer.
An in vitro model was developed for bovine natural corneal endothelia. The cells were cultured to a confluent monolayer and vitrified using 25% (w/w) 1,2-propanediol-35% (w/w) trehalose as cryoprotective agents. Approximately, 61.3% of the cells were viable using the protocol.
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2008
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