The isolation of nuclei containing mitotic spindles from the plasmodium of the slime mould Physarum polycephalum.
We have developed a protocol which facilitates the isolation of nuclei containing mitotic spindles from the plasmodium of Physarum polycephalum. The nuclei are isolated in a spindle stabilization buffer (SSB) consisting of -4 M glycerol, 5 mM EGTA, 10 mM MgCl2, 10 mM PIPES and 0.1% (v/v) Triton X-10...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1984
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