The isolation of nuclei containing mitotic spindles from the plasmodium of the slime mould Physarum polycephalum.
We have developed a protocol which facilitates the isolation of nuclei containing mitotic spindles from the plasmodium of Physarum polycephalum. The nuclei are isolated in a spindle stabilization buffer (SSB) consisting of -4 M glycerol, 5 mM EGTA, 10 mM MgCl2, 10 mM PIPES and 0.1% (v/v) Triton X-10...
मुख्य लेखकों: | Roobol, A, Paul, E, Gull, K |
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स्वरूप: | Journal article |
भाषा: | English |
प्रकाशित: |
1984
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समान संसाधन
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Identification of tubulin isoforms in the plasmodium of Physarum polycephalum by in vitro microtubule assembly.
द्वारा: Roobol, A, और अन्य
प्रकाशित: (1984) -
ISOLATION AND CHARACTERIZATION OF THE MICROTUBULE ORGANIZING CENTER OF PHYSARUM-POLYCEPHALUM MYXAMOEBAE
द्वारा: Clayton, L, और अन्य
प्रकाशित: (1982) -
Location of a single beta-tubulin gene product in both cytoskeletal and mitotic-spindle microtubules in Physarum polycephalum.
द्वारा: Paul, E, और अन्य
प्रकाशित: (1989) -
THE MICROTUBULE-ORGANIZING CENTER OF MYXAMOEBAE OF PHYSARUM-POLYCEPHALUM
द्वारा: Havercroft, J, और अन्य
प्रकाशित: (1981) -
Microtubule nucleation by the isolated microtubule-organizing centre of Physarum polycephalum myxamoebae.
द्वारा: Roobol, A, और अन्य
प्रकाशित: (1982)