The isolation of nuclei containing mitotic spindles from the plasmodium of the slime mould Physarum polycephalum.

We have developed a protocol which facilitates the isolation of nuclei containing mitotic spindles from the plasmodium of Physarum polycephalum. The nuclei are isolated in a spindle stabilization buffer (SSB) consisting of -4 M glycerol, 5 mM EGTA, 10 mM MgCl2, 10 mM PIPES and 0.1% (v/v) Triton X-10...

पूर्ण विवरण

ग्रंथसूची विवरण
मुख्य लेखकों: Roobol, A, Paul, E, Gull, K
स्वरूप: Journal article
भाषा:English
प्रकाशित: 1984

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