The isolation of nuclei containing mitotic spindles from the plasmodium of the slime mould Physarum polycephalum.

We have developed a protocol which facilitates the isolation of nuclei containing mitotic spindles from the plasmodium of Physarum polycephalum. The nuclei are isolated in a spindle stabilization buffer (SSB) consisting of -4 M glycerol, 5 mM EGTA, 10 mM MgCl2, 10 mM PIPES and 0.1% (v/v) Triton X-10...

詳細記述

書誌詳細
主要な著者: Roobol, A, Paul, E, Gull, K
フォーマット: Journal article
言語:English
出版事項: 1984