Proximal promoter elements of the human zeta-globin gene confer embryonic-specific expression on a linked reporter gene in transgenic mice.
We have investigated the transcriptional regulation of the human embryonic zeta-globin gene promoter. First, we examined the effect that deletion of sequences 5' to zeta-globin's CCAAT box have on zeta-promoter activity in erythroid cell lines. Deletions of sequences between -116 and -556...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
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1996
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| _version_ | 1826293457301274624 |
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| author | Pondel, MD Sharpe, J Clark, S Pearson, L Wood, W Proudfoot, N |
| author_facet | Pondel, MD Sharpe, J Clark, S Pearson, L Wood, W Proudfoot, N |
| author_sort | Pondel, MD |
| collection | OXFORD |
| description | We have investigated the transcriptional regulation of the human embryonic zeta-globin gene promoter. First, we examined the effect that deletion of sequences 5' to zeta-globin's CCAAT box have on zeta-promoter activity in erythroid cell lines. Deletions of sequences between -116 and -556 (cap = 0) had little effect while further deletion to -84 reduced zeta-promoter activity by only 2-3-fold in both transiently and stably transfected erythroid cells. Constructs containing 67, 84 and 556 bp of zeta-globin 5' flanking region linked to a beta-galactosidase reporter gene (lacZ) and hypersensitive site -40 (HS-40) of the human alpha-globin gene cluster were then employed for the generation of transgenic mice. LacZ expression from all constructs, including a 67 bp zeta-globin promoter, was erythroid-specific and most active between 8.5 and 10.5 days post-fertilisation. By 16.5 days gestation, lacZ expression dropped 40-100-fold. These results suggest that embryonic-specific activation of the human zeta-globin promoter is conferred by a 67 bp zeta-promoter fragment containing only a CCAAT and TATA box. |
| first_indexed | 2024-03-07T03:30:23Z |
| format | Journal article |
| id | oxford-uuid:ba83d2cf-5d5d-497b-a7a2-7e5a7af7a529 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:30:23Z |
| publishDate | 1996 |
| record_format | dspace |
| spelling | oxford-uuid:ba83d2cf-5d5d-497b-a7a2-7e5a7af7a5292022-03-27T05:10:22ZProximal promoter elements of the human zeta-globin gene confer embryonic-specific expression on a linked reporter gene in transgenic mice.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ba83d2cf-5d5d-497b-a7a2-7e5a7af7a529EnglishSymplectic Elements at Oxford1996Pondel, MDSharpe, JClark, SPearson, LWood, WProudfoot, NWe have investigated the transcriptional regulation of the human embryonic zeta-globin gene promoter. First, we examined the effect that deletion of sequences 5' to zeta-globin's CCAAT box have on zeta-promoter activity in erythroid cell lines. Deletions of sequences between -116 and -556 (cap = 0) had little effect while further deletion to -84 reduced zeta-promoter activity by only 2-3-fold in both transiently and stably transfected erythroid cells. Constructs containing 67, 84 and 556 bp of zeta-globin 5' flanking region linked to a beta-galactosidase reporter gene (lacZ) and hypersensitive site -40 (HS-40) of the human alpha-globin gene cluster were then employed for the generation of transgenic mice. LacZ expression from all constructs, including a 67 bp zeta-globin promoter, was erythroid-specific and most active between 8.5 and 10.5 days post-fertilisation. By 16.5 days gestation, lacZ expression dropped 40-100-fold. These results suggest that embryonic-specific activation of the human zeta-globin promoter is conferred by a 67 bp zeta-promoter fragment containing only a CCAAT and TATA box. |
| spellingShingle | Pondel, MD Sharpe, J Clark, S Pearson, L Wood, W Proudfoot, N Proximal promoter elements of the human zeta-globin gene confer embryonic-specific expression on a linked reporter gene in transgenic mice. |
| title | Proximal promoter elements of the human zeta-globin gene confer embryonic-specific expression on a linked reporter gene in transgenic mice. |
| title_full | Proximal promoter elements of the human zeta-globin gene confer embryonic-specific expression on a linked reporter gene in transgenic mice. |
| title_fullStr | Proximal promoter elements of the human zeta-globin gene confer embryonic-specific expression on a linked reporter gene in transgenic mice. |
| title_full_unstemmed | Proximal promoter elements of the human zeta-globin gene confer embryonic-specific expression on a linked reporter gene in transgenic mice. |
| title_short | Proximal promoter elements of the human zeta-globin gene confer embryonic-specific expression on a linked reporter gene in transgenic mice. |
| title_sort | proximal promoter elements of the human zeta globin gene confer embryonic specific expression on a linked reporter gene in transgenic mice |
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