Cleavage of fragments containing DNA mismatches by enzymic and chemical probes.
We prepared synthetic 50-mer DNA duplexes, each containing four mismatched base-pairs in similar positions. We examined their cleavage by DNases I and II, micrococcal nuclease (MNase), methidiumpropyl-EDTA-Fe(II) [MPE-Fe(II)] and hydroxyl radicals. We find that single mismatches only produce subtle...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2003
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