Expression of protein complexes using multiple Escherichia coli protein co-expression systems: a benchmarking study.

Escherichia coli (E. coli) remains the most commonly used host for recombinant protein expression. It is well known that a variety of experimental factors influence the protein production level as well as the solubility profile of over-expressed proteins. This becomes increasingly important for opti...

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Bibliographic Details
Main Authors: Busso, D, Peleg, Y, Heidebrecht, T, Romier, C, Jacobovitch, Y, Dantes, A, Salim, L, Troesch, E, Schuetz, A, Heinemann, U, Folkers, G, Geerlof, A, Wilmanns, M, Polewacz, A, Quedenau, C, Büssow, K, Adamson, R, Blagova, E, Walton, J, Cartwright, J, Bird, L, Owens, R, Berrow, N, Wilson, K, Sussman, J
Format: Journal article
Language:English
Published: 2011