STED nanoscopy with time-gated detection: theoretical and experimental aspects
In a stimulated emission depletion (STED) microscope the region in which fluorescence markers can emit spontaneously shrinks with continued STED beam action after a singular excitation event. This fact has been recently used to substantially improve the effective spatial resolution in STED nanoscopy...
Main Authors: | , , , , , , |
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Format: | Journal article |
Language: | English |
Published: |
Public Library of Science
2013
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