STED nanoscopy with time-gated detection: theoretical and experimental aspects

In a stimulated emission depletion (STED) microscope the region in which fluorescence markers can emit spontaneously shrinks with continued STED beam action after a singular excitation event. This fact has been recently used to substantially improve the effective spatial resolution in STED nanoscopy...

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Bibliographic Details
Main Authors: Vicidomini, G, Schönle, A, Ta, H, Han, K, Moneron, G, Eggeling, C, Hell, S
Format: Journal article
Language:English
Published: Public Library of Science 2013