Yeast artificial chromosome libraries containing large inserts from mouse and human DNA.
Yeast artificial chromosome (YAC) libraries have been difficult to construct with average insert sizes greater than 400 kilobase pairs when DNA is size-fractionated in low-melting-point agarose. By using yeast chromosome in mock cloning experiments, we found that polyamines should be present wheneve...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1991
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