The assembly of large BACs by in vivo recombination.
We have developed a method for recombining bacterial artificial chromosomes (BACs) and P1 artificial chromosomes (PACs) containing large genomic DNA fragments into a single vector using the Cre-lox recombination system from bacteriophage P1 in vivo. This overcomes the limitations of in vitro methods...
| Main Authors: | , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2000
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