Structures of SAS-6 suggest its organization in centrioles.

Structures of SAS-6 suggest its organization in centrioles.

Centrioles are cylindrical, ninefold symmetrical structures with peripheral triplet microtubules strictly required to template cilia and flagella. The highly conserved protein SAS-6 constitutes the center of the cartwheel assembly that scaffolds centrioles early in their biogenesis. We determined th...

Full description

Bibliographic Details
Main Authors: van Breugel, M, Hirono, M, Andreeva, A, Yanagisawa, H, Yamaguchi, S, Nakazawa, Y, Morgner, N, Petrovich, M, Ebong, I, Robinson, C, Johnson, C, Veprintsev, D, Zuber, B
Format: Journal article
Language:English
Published: 2011
Description
Summary:Centrioles are cylindrical, ninefold symmetrical structures with peripheral triplet microtubules strictly required to template cilia and flagella. The highly conserved protein SAS-6 constitutes the center of the cartwheel assembly that scaffolds centrioles early in their biogenesis. We determined the x-ray structure of the amino-terminal domain of SAS-6 from zebrafish, and we show that recombinant SAS-6 self-associates in vitro into assemblies that resemble cartwheel centers. Point mutations are consistent with the notion that centriole formation in vivo depends on the interactions that define the self-assemblies observed here. Thus, these interactions are probably essential to the structural organization of cartwheel centers.

Similar Items