Structures of SAS-6 suggest its organization in centrioles.
Centrioles are cylindrical, ninefold symmetrical structures with peripheral triplet microtubules strictly required to template cilia and flagella. The highly conserved protein SAS-6 constitutes the center of the cartwheel assembly that scaffolds centrioles early in their biogenesis. We determined th...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2011
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| _version_ | 1797092561537466368 |
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| author | van Breugel, M Hirono, M Andreeva, A Yanagisawa, H Yamaguchi, S Nakazawa, Y Morgner, N Petrovich, M Ebong, I Robinson, C Johnson, C Veprintsev, D Zuber, B |
| author_facet | van Breugel, M Hirono, M Andreeva, A Yanagisawa, H Yamaguchi, S Nakazawa, Y Morgner, N Petrovich, M Ebong, I Robinson, C Johnson, C Veprintsev, D Zuber, B |
| author_sort | van Breugel, M |
| collection | OXFORD |
| description | Centrioles are cylindrical, ninefold symmetrical structures with peripheral triplet microtubules strictly required to template cilia and flagella. The highly conserved protein SAS-6 constitutes the center of the cartwheel assembly that scaffolds centrioles early in their biogenesis. We determined the x-ray structure of the amino-terminal domain of SAS-6 from zebrafish, and we show that recombinant SAS-6 self-associates in vitro into assemblies that resemble cartwheel centers. Point mutations are consistent with the notion that centriole formation in vivo depends on the interactions that define the self-assemblies observed here. Thus, these interactions are probably essential to the structural organization of cartwheel centers. |
| first_indexed | 2024-03-07T03:47:43Z |
| format | Journal article |
| id | oxford-uuid:c0179c10-9e27-48c3-8781-f3e3450f1ac9 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:47:43Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oxford-uuid:c0179c10-9e27-48c3-8781-f3e3450f1ac92022-03-27T05:52:10ZStructures of SAS-6 suggest its organization in centrioles.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c0179c10-9e27-48c3-8781-f3e3450f1ac9EnglishSymplectic Elements at Oxford2011van Breugel, MHirono, MAndreeva, AYanagisawa, HYamaguchi, SNakazawa, YMorgner, NPetrovich, MEbong, IRobinson, CJohnson, CVeprintsev, DZuber, BCentrioles are cylindrical, ninefold symmetrical structures with peripheral triplet microtubules strictly required to template cilia and flagella. The highly conserved protein SAS-6 constitutes the center of the cartwheel assembly that scaffolds centrioles early in their biogenesis. We determined the x-ray structure of the amino-terminal domain of SAS-6 from zebrafish, and we show that recombinant SAS-6 self-associates in vitro into assemblies that resemble cartwheel centers. Point mutations are consistent with the notion that centriole formation in vivo depends on the interactions that define the self-assemblies observed here. Thus, these interactions are probably essential to the structural organization of cartwheel centers. |
| spellingShingle | van Breugel, M Hirono, M Andreeva, A Yanagisawa, H Yamaguchi, S Nakazawa, Y Morgner, N Petrovich, M Ebong, I Robinson, C Johnson, C Veprintsev, D Zuber, B Structures of SAS-6 suggest its organization in centrioles. |
| title | Structures of SAS-6 suggest its organization in centrioles. |
| title_full | Structures of SAS-6 suggest its organization in centrioles. |
| title_fullStr | Structures of SAS-6 suggest its organization in centrioles. |
| title_full_unstemmed | Structures of SAS-6 suggest its organization in centrioles. |
| title_short | Structures of SAS-6 suggest its organization in centrioles. |
| title_sort | structures of sas 6 suggest its organization in centrioles |
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