Resolution of lambda/10 in fluorescence microscopy using fast single molecule photo-switching

We demonstrate nanoscale resolution in far-field optical microscopy based on photo-switching of molecules. By enabling, recording and disabling fluorescence from individual labels sequentially, the detection volume is reduced to the size of a single molecule and the diffraction limit is broken. Imag...

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Main Authors: Geisler, C, Schoenle, A, von Middendorff, C, Bock, H, Eggeling, C, Egner, A, Hell, S
格式: Journal article
语言:English
出版: 2007