Resolution of lambda/10 in fluorescence microscopy using fast single molecule photo-switching
We demonstrate nanoscale resolution in far-field optical microscopy based on photo-switching of molecules. By enabling, recording and disabling fluorescence from individual labels sequentially, the detection volume is reduced to the size of a single molecule and the diffraction limit is broken. Imag...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2007
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| _version_ | 1826294623532744704 |
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| author | Geisler, C Schoenle, A von Middendorff, C Bock, H Eggeling, C Egner, A Hell, S |
| author_facet | Geisler, C Schoenle, A von Middendorff, C Bock, H Eggeling, C Egner, A Hell, S |
| author_sort | Geisler, C |
| collection | OXFORD |
| description | We demonstrate nanoscale resolution in far-field optical microscopy based on photo-switching of molecules. By enabling, recording and disabling fluorescence from individual labels sequentially, the detection volume is reduced to the size of a single molecule and the diffraction limit is broken. Images of nanostructures milled into a coverslip and tagged by fluorescent proteins could be recorded at 50 nm resolution. Due to the fast and asynchronous image acquisition protocol used in these experiments, we were able to reduce acquisition times to ∼ 2.5 min, which is two orders of magnitude lower than in previous implementations. © Springer-Verlag 2007. |
| first_indexed | 2024-03-07T03:48:32Z |
| format | Journal article |
| id | oxford-uuid:c063d22b-d179-4825-90d1-1b2f48bbb5d2 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:48:32Z |
| publishDate | 2007 |
| record_format | dspace |
| spelling | oxford-uuid:c063d22b-d179-4825-90d1-1b2f48bbb5d22022-03-27T05:54:03ZResolution of lambda/10 in fluorescence microscopy using fast single molecule photo-switchingJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c063d22b-d179-4825-90d1-1b2f48bbb5d2EnglishSymplectic Elements at Oxford2007Geisler, CSchoenle, Avon Middendorff, CBock, HEggeling, CEgner, AHell, SWe demonstrate nanoscale resolution in far-field optical microscopy based on photo-switching of molecules. By enabling, recording and disabling fluorescence from individual labels sequentially, the detection volume is reduced to the size of a single molecule and the diffraction limit is broken. Images of nanostructures milled into a coverslip and tagged by fluorescent proteins could be recorded at 50 nm resolution. Due to the fast and asynchronous image acquisition protocol used in these experiments, we were able to reduce acquisition times to ∼ 2.5 min, which is two orders of magnitude lower than in previous implementations. © Springer-Verlag 2007. |
| spellingShingle | Geisler, C Schoenle, A von Middendorff, C Bock, H Eggeling, C Egner, A Hell, S Resolution of lambda/10 in fluorescence microscopy using fast single molecule photo-switching |
| title | Resolution of lambda/10 in fluorescence microscopy using fast single molecule photo-switching |
| title_full | Resolution of lambda/10 in fluorescence microscopy using fast single molecule photo-switching |
| title_fullStr | Resolution of lambda/10 in fluorescence microscopy using fast single molecule photo-switching |
| title_full_unstemmed | Resolution of lambda/10 in fluorescence microscopy using fast single molecule photo-switching |
| title_short | Resolution of lambda/10 in fluorescence microscopy using fast single molecule photo-switching |
| title_sort | resolution of lambda 10 in fluorescence microscopy using fast single molecule photo switching |
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