Comparison of a limiting dilution assay and ELISpot for detection of memory B-cells before and after immunisation with a protein-polysaccharide conjugate vaccine in children
In the present study, the limiting dilution assay (LDA) and the ELISpot are compared in their ability to detect serogroup C meningococcal (MenC)-specific memory B-cells in peripheral blood of 12 month old children, after 3-dose priming with serogroup C meningococcal conjugate vaccine (MenCV) in infa...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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Elsevier
2010
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| _version_ | 1826294647242096640 |
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| author | Blanchard-Rohner, G Galli, G Clutterbuck, E Pollard, A |
| author2 | Association of Medical Laboratory Immunologists |
| author_facet | Association of Medical Laboratory Immunologists Blanchard-Rohner, G Galli, G Clutterbuck, E Pollard, A |
| author_sort | Blanchard-Rohner, G |
| collection | OXFORD |
| description | In the present study, the limiting dilution assay (LDA) and the ELISpot are compared in their ability to detect serogroup C meningococcal (MenC)-specific memory B-cells in peripheral blood of 12 month old children, after 3-dose priming with serogroup C meningococcal conjugate vaccine (MenCV) in infancy. At 12 months of age, MenC-memory B-cells were detected by ELISpt in 61% of children and in 5% by LDA. In contrast, carrier-specific memory B-cells were measurable in 36% of children by LDA and 78% by ELISpot. One month after a booster dose of MenCV given at 12 months of age, MenC-specific memory B-cells were detected in 89% of children by ELISpot and 65% of children by LDA, while diphtheria toxoid-specific memory B-cells were detected in 88% of childen by ELISpot and in 74% of children by LDA. Two statistical methods were applied to enumerate memory B-cells with the LDA assay; the Poisson method allowed determination of very low frequencies that could not be determined by the Reed and Muench method. These data are examples of alternative methods to assess long-term protection after protein-polysaccharide conjugate vaccine, through direct measurement of memory B-cells in peripheral blood shortly after immunisation. |
| first_indexed | 2024-03-07T03:48:54Z |
| format | Journal article |
| id | oxford-uuid:c084da36-f13d-4d99-bfef-9a741e1f971c |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:48:54Z |
| publishDate | 2010 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:c084da36-f13d-4d99-bfef-9a741e1f971c2022-03-27T05:54:59ZComparison of a limiting dilution assay and ELISpot for detection of memory B-cells before and after immunisation with a protein-polysaccharide conjugate vaccine in childrenJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c084da36-f13d-4d99-bfef-9a741e1f971cVaccinologyImmunologyPaediatricsEnglishOxford University Research Archive - ValetElsevier2010Blanchard-Rohner, GGalli, GClutterbuck, EPollard, AAssociation of Medical Laboratory ImmunologistsIn the present study, the limiting dilution assay (LDA) and the ELISpot are compared in their ability to detect serogroup C meningococcal (MenC)-specific memory B-cells in peripheral blood of 12 month old children, after 3-dose priming with serogroup C meningococcal conjugate vaccine (MenCV) in infancy. At 12 months of age, MenC-memory B-cells were detected by ELISpt in 61% of children and in 5% by LDA. In contrast, carrier-specific memory B-cells were measurable in 36% of children by LDA and 78% by ELISpot. One month after a booster dose of MenCV given at 12 months of age, MenC-specific memory B-cells were detected in 89% of children by ELISpot and 65% of children by LDA, while diphtheria toxoid-specific memory B-cells were detected in 88% of childen by ELISpot and in 74% of children by LDA. Two statistical methods were applied to enumerate memory B-cells with the LDA assay; the Poisson method allowed determination of very low frequencies that could not be determined by the Reed and Muench method. These data are examples of alternative methods to assess long-term protection after protein-polysaccharide conjugate vaccine, through direct measurement of memory B-cells in peripheral blood shortly after immunisation. |
| spellingShingle | Vaccinology Immunology Paediatrics Blanchard-Rohner, G Galli, G Clutterbuck, E Pollard, A Comparison of a limiting dilution assay and ELISpot for detection of memory B-cells before and after immunisation with a protein-polysaccharide conjugate vaccine in children |
| title | Comparison of a limiting dilution assay and ELISpot for detection of memory B-cells before and after immunisation with a protein-polysaccharide conjugate vaccine in children |
| title_full | Comparison of a limiting dilution assay and ELISpot for detection of memory B-cells before and after immunisation with a protein-polysaccharide conjugate vaccine in children |
| title_fullStr | Comparison of a limiting dilution assay and ELISpot for detection of memory B-cells before and after immunisation with a protein-polysaccharide conjugate vaccine in children |
| title_full_unstemmed | Comparison of a limiting dilution assay and ELISpot for detection of memory B-cells before and after immunisation with a protein-polysaccharide conjugate vaccine in children |
| title_short | Comparison of a limiting dilution assay and ELISpot for detection of memory B-cells before and after immunisation with a protein-polysaccharide conjugate vaccine in children |
| title_sort | comparison of a limiting dilution assay and elispot for detection of memory b cells before and after immunisation with a protein polysaccharide conjugate vaccine in children |
| topic | Vaccinology Immunology Paediatrics |
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