Comparison of a limiting dilution assay and ELISpot for detection of memory B-cells before and after immunisation with a protein-polysaccharide conjugate vaccine in children

In the present study, the limiting dilution assay (LDA) and the ELISpot are compared in their ability to detect serogroup C meningococcal (MenC)-specific memory B-cells in peripheral blood of 12 month old children, after 3-dose priming with serogroup C meningococcal conjugate vaccine (MenCV) in infa...

Ամբողջական նկարագրություն

Մատենագիտական մանրամասներ
Հիմնական հեղինակներ: Blanchard-Rohner, G, Galli, G, Clutterbuck, E, Pollard, A
Այլ հեղինակներ: Association of Medical Laboratory Immunologists
Ձևաչափ: Journal article
Լեզու:English
Հրապարակվել է: Elsevier 2010
Խորագրեր:
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author Blanchard-Rohner, G
Galli, G
Clutterbuck, E
Pollard, A
author2 Association of Medical Laboratory Immunologists
author_facet Association of Medical Laboratory Immunologists
Blanchard-Rohner, G
Galli, G
Clutterbuck, E
Pollard, A
author_sort Blanchard-Rohner, G
collection OXFORD
description In the present study, the limiting dilution assay (LDA) and the ELISpot are compared in their ability to detect serogroup C meningococcal (MenC)-specific memory B-cells in peripheral blood of 12 month old children, after 3-dose priming with serogroup C meningococcal conjugate vaccine (MenCV) in infancy. At 12 months of age, MenC-memory B-cells were detected by ELISpt in 61% of children and in 5% by LDA. In contrast, carrier-specific memory B-cells were measurable in 36% of children by LDA and 78% by ELISpot. One month after a booster dose of MenCV given at 12 months of age, MenC-specific memory B-cells were detected in 89% of children by ELISpot and 65% of children by LDA, while diphtheria toxoid-specific memory B-cells were detected in 88% of childen by ELISpot and in 74% of children by LDA. Two statistical methods were applied to enumerate memory B-cells with the LDA assay; the Poisson method allowed determination of very low frequencies that could not be determined by the Reed and Muench method. These data are examples of alternative methods to assess long-term protection after protein-polysaccharide conjugate vaccine, through direct measurement of memory B-cells in peripheral blood shortly after immunisation.
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spelling oxford-uuid:c084da36-f13d-4d99-bfef-9a741e1f971c2022-03-27T05:54:59ZComparison of a limiting dilution assay and ELISpot for detection of memory B-cells before and after immunisation with a protein-polysaccharide conjugate vaccine in childrenJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c084da36-f13d-4d99-bfef-9a741e1f971cVaccinologyImmunologyPaediatricsEnglishOxford University Research Archive - ValetElsevier2010Blanchard-Rohner, GGalli, GClutterbuck, EPollard, AAssociation of Medical Laboratory ImmunologistsIn the present study, the limiting dilution assay (LDA) and the ELISpot are compared in their ability to detect serogroup C meningococcal (MenC)-specific memory B-cells in peripheral blood of 12 month old children, after 3-dose priming with serogroup C meningococcal conjugate vaccine (MenCV) in infancy. At 12 months of age, MenC-memory B-cells were detected by ELISpt in 61% of children and in 5% by LDA. In contrast, carrier-specific memory B-cells were measurable in 36% of children by LDA and 78% by ELISpot. One month after a booster dose of MenCV given at 12 months of age, MenC-specific memory B-cells were detected in 89% of children by ELISpot and 65% of children by LDA, while diphtheria toxoid-specific memory B-cells were detected in 88% of childen by ELISpot and in 74% of children by LDA. Two statistical methods were applied to enumerate memory B-cells with the LDA assay; the Poisson method allowed determination of very low frequencies that could not be determined by the Reed and Muench method. These data are examples of alternative methods to assess long-term protection after protein-polysaccharide conjugate vaccine, through direct measurement of memory B-cells in peripheral blood shortly after immunisation.
spellingShingle Vaccinology
Immunology
Paediatrics
Blanchard-Rohner, G
Galli, G
Clutterbuck, E
Pollard, A
Comparison of a limiting dilution assay and ELISpot for detection of memory B-cells before and after immunisation with a protein-polysaccharide conjugate vaccine in children
title Comparison of a limiting dilution assay and ELISpot for detection of memory B-cells before and after immunisation with a protein-polysaccharide conjugate vaccine in children
title_full Comparison of a limiting dilution assay and ELISpot for detection of memory B-cells before and after immunisation with a protein-polysaccharide conjugate vaccine in children
title_fullStr Comparison of a limiting dilution assay and ELISpot for detection of memory B-cells before and after immunisation with a protein-polysaccharide conjugate vaccine in children
title_full_unstemmed Comparison of a limiting dilution assay and ELISpot for detection of memory B-cells before and after immunisation with a protein-polysaccharide conjugate vaccine in children
title_short Comparison of a limiting dilution assay and ELISpot for detection of memory B-cells before and after immunisation with a protein-polysaccharide conjugate vaccine in children
title_sort comparison of a limiting dilution assay and elispot for detection of memory b cells before and after immunisation with a protein polysaccharide conjugate vaccine in children
topic Vaccinology
Immunology
Paediatrics
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