Quantitative in vivo measurement of glutathione in Arabidopsis cells.
A new, non-destructive assay is described to quantify cytoplasmic glutathione (GSH) levels in vivo in single cells or populations of cells from Arabidopsis suspension cultures. Cytoplasmic GSH was labelled with monochlorobimane (MCB) in situ to give a fluorescent GSH-bimane (GSB) conjugate. At low (...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2001
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