Multiplexing PKA and ERK1&2 kinases FRET biosensors in living cells using single excitation wavelength dual colour FLIM

Monitoring of different signalling enzymes in a single assay using multiplex biosensing provides a multidimensional workspace to elucidate biological processes, signalling pathway crosstalk, and determine precise sequence of events at the single living cell level. In this study, we interrogate the c...

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Bibliographic Details
Main Authors: Demeautis, C, Sipieter, F, Roul, J, Chapuis, C, Padilla-Parra, S, Riquet, F, Tramier, M
Format: Journal article
Published: Nature Publishing Group 2017