TARGET-Seq: a protocol for high-sensitivity single-cell mutational analysis and parallel RNA sequencing
Single-cell RNA-sequencing technologies are ideally placed to resolve intratumoral heterogeneity. However, the lack of coverage across key mutation hotspots has precluded the correlation of genetic and transcriptional readouts from the same single cell. To overcome this, we developed TARGET-seq, a p...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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Cell Press
2020
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| _version_ | 1826295196291170304 |
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| author | Rodriguez-Meira, A O'Sullivan, J Rahman, H Mead, AJ |
| author_facet | Rodriguez-Meira, A O'Sullivan, J Rahman, H Mead, AJ |
| author_sort | Rodriguez-Meira, A |
| collection | OXFORD |
| description | Single-cell RNA-sequencing technologies are ideally placed to resolve intratumoral heterogeneity. However, the lack of coverage across key mutation hotspots has precluded the correlation of genetic and transcriptional readouts from the same single cell. To overcome this, we developed TARGET-seq, a protocol for TARGETed high-sensitivity single-cell mutational analysis with extremely low allelic dropout rates, parallel RNA SEQuencing, and cell-surface proteomics. Here, we present a detailed step-by-step protocol for TARGET-seq, including troubleshooting tips, approaches for automation, and methods for high-throughput multiplexing of libraries. For complete details on the use and execution of this protocol, please refer to Rodriguez-Meira et al. (2019). |
| first_indexed | 2024-03-07T03:57:19Z |
| format | Journal article |
| id | oxford-uuid:c3514235-208e-4a9d-8ed9-6c5a361be0b2 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:57:19Z |
| publishDate | 2020 |
| publisher | Cell Press |
| record_format | dspace |
| spelling | oxford-uuid:c3514235-208e-4a9d-8ed9-6c5a361be0b22022-03-27T06:15:35ZTARGET-Seq: a protocol for high-sensitivity single-cell mutational analysis and parallel RNA sequencing Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c3514235-208e-4a9d-8ed9-6c5a361be0b2EnglishSymplectic ElementsCell Press2020Rodriguez-Meira, AO'Sullivan, JRahman, HMead, AJSingle-cell RNA-sequencing technologies are ideally placed to resolve intratumoral heterogeneity. However, the lack of coverage across key mutation hotspots has precluded the correlation of genetic and transcriptional readouts from the same single cell. To overcome this, we developed TARGET-seq, a protocol for TARGETed high-sensitivity single-cell mutational analysis with extremely low allelic dropout rates, parallel RNA SEQuencing, and cell-surface proteomics. Here, we present a detailed step-by-step protocol for TARGET-seq, including troubleshooting tips, approaches for automation, and methods for high-throughput multiplexing of libraries. For complete details on the use and execution of this protocol, please refer to Rodriguez-Meira et al. (2019). |
| spellingShingle | Rodriguez-Meira, A O'Sullivan, J Rahman, H Mead, AJ TARGET-Seq: a protocol for high-sensitivity single-cell mutational analysis and parallel RNA sequencing |
| title | TARGET-Seq: a protocol for high-sensitivity single-cell mutational analysis and parallel RNA sequencing |
| title_full | TARGET-Seq: a protocol for high-sensitivity single-cell mutational analysis and parallel RNA sequencing |
| title_fullStr | TARGET-Seq: a protocol for high-sensitivity single-cell mutational analysis and parallel RNA sequencing |
| title_full_unstemmed | TARGET-Seq: a protocol for high-sensitivity single-cell mutational analysis and parallel RNA sequencing |
| title_short | TARGET-Seq: a protocol for high-sensitivity single-cell mutational analysis and parallel RNA sequencing |
| title_sort | target seq a protocol for high sensitivity single cell mutational analysis and parallel rna sequencing |
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