Duplex Scorpion primers in SNP analysis and FRET applications.
Scorpions are fluorogenic PCR primers with a probe element attached at the 5'-end via a PCR stopper. They are used in real-time amplicon-specific detection of PCR products in homogeneous solution. Two different formats are possible, the 'stem-loop' format and the 'duplex' fo...
Main Authors: | , , , , , , |
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Format: | Journal article |
Language: | English |
Published: |
2001
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