Duplex Scorpion primers in SNP analysis and FRET applications.

Scorpions are fluorogenic PCR primers with a probe element attached at the 5'-end via a PCR stopper. They are used in real-time amplicon-specific detection of PCR products in homogeneous solution. Two different formats are possible, the 'stem-loop' format and the 'duplex' fo...

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Bibliographic Details
Main Authors: Solinas, A, Brown, L, McKeen, C, Mellor, J, Nicol, J, Thelwell, N, Brown, T
Format: Journal article
Language:English
Published: 2001