Epithelial cells bearing class II molecules stimulate allogeneic human colonic intraepithelial lymphocytes.
HLA-DR+ gut epithelial cells may present antigen to intraepithelial lymphocytes (IEL). This study aimed to isolate an IEL population from the human colon to activate CD3 + IEL by a human colonic epithelial cell line (HT-29), bearing different concentrations of class II antigen (HLA-DR). IEL were iso...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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BMJ Publishing Group
1992
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| _version_ | 1797093439288901632 |
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| author | Hoang, P Crotty, B Dalton, H Jewell, D |
| author_facet | Hoang, P Crotty, B Dalton, H Jewell, D |
| author_sort | Hoang, P |
| collection | OXFORD |
| description | HLA-DR+ gut epithelial cells may present antigen to intraepithelial lymphocytes (IEL). This study aimed to isolate an IEL population from the human colon to activate CD3 + IEL by a human colonic epithelial cell line (HT-29), bearing different concentrations of class II antigen (HLA-DR). IEL were isolated by a mechanical method from six patients with ulcerative colitis (UC) and from 14 control patients. IEL were cocultured with HT-29 which had been induced to express class II molecules by gamma-interferon (IFN-gamma) in a dose dependent manner. The phenotype and the subsequent expression of activation markers by the IEL were determined to two colour flow cytometry. The IEL population had a CD4/CD8 ratio similar to that seen in tissue sections. In the mixed cell culture, the degree of IEL activation showed a positive correlation with the degree of HLA-DR expression by the HT29 cells and the IEL secreted a IFN-gamma like factor that in turn stimulated the HT-29. Thus, depending on their expression of HLA molecules, colonic epithelial cells are able to activate CD3+CD8+IEL. |
| first_indexed | 2024-03-07T04:00:24Z |
| format | Journal article |
| id | oxford-uuid:c4639f70-a8b7-43d3-821d-659aa2cbd3c2 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:00:24Z |
| publishDate | 1992 |
| publisher | BMJ Publishing Group |
| record_format | dspace |
| spelling | oxford-uuid:c4639f70-a8b7-43d3-821d-659aa2cbd3c22022-03-27T06:22:59ZEpithelial cells bearing class II molecules stimulate allogeneic human colonic intraepithelial lymphocytes.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c4639f70-a8b7-43d3-821d-659aa2cbd3c2EnglishSymplectic Elements at OxfordBMJ Publishing Group1992Hoang, PCrotty, BDalton, HJewell, DHLA-DR+ gut epithelial cells may present antigen to intraepithelial lymphocytes (IEL). This study aimed to isolate an IEL population from the human colon to activate CD3 + IEL by a human colonic epithelial cell line (HT-29), bearing different concentrations of class II antigen (HLA-DR). IEL were isolated by a mechanical method from six patients with ulcerative colitis (UC) and from 14 control patients. IEL were cocultured with HT-29 which had been induced to express class II molecules by gamma-interferon (IFN-gamma) in a dose dependent manner. The phenotype and the subsequent expression of activation markers by the IEL were determined to two colour flow cytometry. The IEL population had a CD4/CD8 ratio similar to that seen in tissue sections. In the mixed cell culture, the degree of IEL activation showed a positive correlation with the degree of HLA-DR expression by the HT29 cells and the IEL secreted a IFN-gamma like factor that in turn stimulated the HT-29. Thus, depending on their expression of HLA molecules, colonic epithelial cells are able to activate CD3+CD8+IEL. |
| spellingShingle | Hoang, P Crotty, B Dalton, H Jewell, D Epithelial cells bearing class II molecules stimulate allogeneic human colonic intraepithelial lymphocytes. |
| title | Epithelial cells bearing class II molecules stimulate allogeneic human colonic intraepithelial lymphocytes. |
| title_full | Epithelial cells bearing class II molecules stimulate allogeneic human colonic intraepithelial lymphocytes. |
| title_fullStr | Epithelial cells bearing class II molecules stimulate allogeneic human colonic intraepithelial lymphocytes. |
| title_full_unstemmed | Epithelial cells bearing class II molecules stimulate allogeneic human colonic intraepithelial lymphocytes. |
| title_short | Epithelial cells bearing class II molecules stimulate allogeneic human colonic intraepithelial lymphocytes. |
| title_sort | epithelial cells bearing class ii molecules stimulate allogeneic human colonic intraepithelial lymphocytes |
| work_keys_str_mv | AT hoangp epithelialcellsbearingclassiimoleculesstimulateallogeneichumancolonicintraepitheliallymphocytes AT crottyb epithelialcellsbearingclassiimoleculesstimulateallogeneichumancolonicintraepitheliallymphocytes AT daltonh epithelialcellsbearingclassiimoleculesstimulateallogeneichumancolonicintraepitheliallymphocytes AT jewelld epithelialcellsbearingclassiimoleculesstimulateallogeneichumancolonicintraepitheliallymphocytes |