Precision modulation of neurodegenerative disease-related gene expression in human iPSC-derived neurons
The ability to reprogram adult somatic cells into induced pluripotent stem cells (iPSCs) and the subsequent development of protocols for their differentiation into disease-relevant cell types have enabled in-depth molecular analyses of multiple disease states as hitherto impossible. Neurons differen...
| Główni autorzy: | , , |
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| Format: | Journal article |
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Springer Nature
2016
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| _version_ | 1826295720697659392 |
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| author | Heman-Ackah, S Bassett, A Wood, M |
| author_facet | Heman-Ackah, S Bassett, A Wood, M |
| author_sort | Heman-Ackah, S |
| collection | OXFORD |
| description | The ability to reprogram adult somatic cells into induced pluripotent stem cells (iPSCs) and the subsequent development of protocols for their differentiation into disease-relevant cell types have enabled in-depth molecular analyses of multiple disease states as hitherto impossible. Neurons differentiated from patient-specific iPSCs provide a means to recapitulate molecular phenotypes of neurodegenerative diseases in vitro. However, it remains challenging to conduct precise manipulations of gene expression in iPSC-derived neurons towards modeling complex human neurological diseases. The application of CRISPR/Cas9 to mammalian systems is revolutionizing the utilization of genome editing technologies in the study of molecular contributors to the pathogenesis of numerous diseases. Here, we demonstrate that CRISPRa and CRISPRi can be used to exert precise modulations of endogenous gene expression in fate-committed iPSC-derived neurons. This highlights CRISPRa/i as a major technical advancement in accessible tools for evaluating the specific contributions of critical neurodegenerative disease-related genes to neuropathogenesis. |
| first_indexed | 2024-03-07T04:05:23Z |
| format | Journal article |
| id | oxford-uuid:c5f6c94b-6598-41f8-ae4c-28b37d9afc2a |
| institution | University of Oxford |
| last_indexed | 2024-03-07T04:05:23Z |
| publishDate | 2016 |
| publisher | Springer Nature |
| record_format | dspace |
| spelling | oxford-uuid:c5f6c94b-6598-41f8-ae4c-28b37d9afc2a2022-03-27T06:34:51ZPrecision modulation of neurodegenerative disease-related gene expression in human iPSC-derived neuronsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c5f6c94b-6598-41f8-ae4c-28b37d9afc2aSymplectic Elements at OxfordSpringer Nature2016Heman-Ackah, SBassett, AWood, MThe ability to reprogram adult somatic cells into induced pluripotent stem cells (iPSCs) and the subsequent development of protocols for their differentiation into disease-relevant cell types have enabled in-depth molecular analyses of multiple disease states as hitherto impossible. Neurons differentiated from patient-specific iPSCs provide a means to recapitulate molecular phenotypes of neurodegenerative diseases in vitro. However, it remains challenging to conduct precise manipulations of gene expression in iPSC-derived neurons towards modeling complex human neurological diseases. The application of CRISPR/Cas9 to mammalian systems is revolutionizing the utilization of genome editing technologies in the study of molecular contributors to the pathogenesis of numerous diseases. Here, we demonstrate that CRISPRa and CRISPRi can be used to exert precise modulations of endogenous gene expression in fate-committed iPSC-derived neurons. This highlights CRISPRa/i as a major technical advancement in accessible tools for evaluating the specific contributions of critical neurodegenerative disease-related genes to neuropathogenesis. |
| spellingShingle | Heman-Ackah, S Bassett, A Wood, M Precision modulation of neurodegenerative disease-related gene expression in human iPSC-derived neurons |
| title | Precision modulation of neurodegenerative disease-related gene expression in human iPSC-derived neurons |
| title_full | Precision modulation of neurodegenerative disease-related gene expression in human iPSC-derived neurons |
| title_fullStr | Precision modulation of neurodegenerative disease-related gene expression in human iPSC-derived neurons |
| title_full_unstemmed | Precision modulation of neurodegenerative disease-related gene expression in human iPSC-derived neurons |
| title_short | Precision modulation of neurodegenerative disease-related gene expression in human iPSC-derived neurons |
| title_sort | precision modulation of neurodegenerative disease related gene expression in human ipsc derived neurons |
| work_keys_str_mv | AT hemanackahs precisionmodulationofneurodegenerativediseaserelatedgeneexpressioninhumanipscderivedneurons AT bassetta precisionmodulationofneurodegenerativediseaserelatedgeneexpressioninhumanipscderivedneurons AT woodm precisionmodulationofneurodegenerativediseaserelatedgeneexpressioninhumanipscderivedneurons |