Three-dimensional adaptive optical nanoscopy for thick specimen imaging at sub-50-nm resolution

Understanding cellular organization demands the best possible spatial resolution in all three dimensions. In fluorescence microscopy, this is achieved by 4Pi nanoscopy methods that combine the concepts of using two opposing objectives for optimal diffraction-limited 3D resolution with switching fluo...

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Bibliographic Details
Main Authors: Hao, X, Allgeyer, ES, Lee, D-R, Antonello, J, Watters, K, Gerdes, JA, Schroeder, LK, Bottanelli, F, Zhao, J, Kidd, P, Lessard, MD, Rothman, JE, Cooley, L, Biederer, T, Booth, MJ, Bewersdorf, J
Format: Journal article
Language:English
Published: Springer Nature 2021