Controlling the mobility of oligonucleotides in the nanochannels of mesoporous silica
Oligonucleotides used in gene therapy and silencing are fragile compounds that degrade easily in biological environments. Porous biocompatible carrier particles may provide a useful strategy to deliver these therapeutics to their target sites. Development of appropriate delivery vehicles, however, r...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2012
|
| _version_ | 1797094058764533760 |
|---|---|
| author | Lebold, T Schlossbauer, A Schneider, K Schermelleh, L Leonhardt, H Bein, T Bräuchle, C |
| author_facet | Lebold, T Schlossbauer, A Schneider, K Schermelleh, L Leonhardt, H Bein, T Bräuchle, C |
| author_sort | Lebold, T |
| collection | OXFORD |
| description | Oligonucleotides used in gene therapy and silencing are fragile compounds that degrade easily in biological environments. Porous biocompatible carrier particles may provide a useful strategy to deliver these therapeutics to their target sites. Development of appropriate delivery vehicles, however, requires a better understanding of the oligonucleotide-host interactions and the oligonucleotide dynamics inside carrier particles. We investigated template-free SBA-15 type mesoporous silica particles and report their loading characteristics with siRNA depending on the surface functionalization of their porous network. We show that the siRNA uptake capability of the particles can be controlled by the composition of the functional groups. Fluorescence recovery after photobleaching measurements revealed size-dependent mobility of siRNA and double-stranded DNA oligonucleotides within the functionalized silica particles and provided evidence for the stability of the oligonucleotides inside the pores. Hence, our study demonstrates the potential of mesoporous silica particles as a means for alternative gene delivery in nanomedicine. Copyright © 2012 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim. |
| first_indexed | 2024-03-07T04:08:52Z |
| format | Journal article |
| id | oxford-uuid:c72424bf-174b-4e89-93b4-2768599847e9 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:08:52Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | oxford-uuid:c72424bf-174b-4e89-93b4-2768599847e92022-03-27T06:42:56ZControlling the mobility of oligonucleotides in the nanochannels of mesoporous silicaJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c72424bf-174b-4e89-93b4-2768599847e9EnglishSymplectic Elements at Oxford2012Lebold, TSchlossbauer, ASchneider, KSchermelleh, LLeonhardt, HBein, TBräuchle, COligonucleotides used in gene therapy and silencing are fragile compounds that degrade easily in biological environments. Porous biocompatible carrier particles may provide a useful strategy to deliver these therapeutics to their target sites. Development of appropriate delivery vehicles, however, requires a better understanding of the oligonucleotide-host interactions and the oligonucleotide dynamics inside carrier particles. We investigated template-free SBA-15 type mesoporous silica particles and report their loading characteristics with siRNA depending on the surface functionalization of their porous network. We show that the siRNA uptake capability of the particles can be controlled by the composition of the functional groups. Fluorescence recovery after photobleaching measurements revealed size-dependent mobility of siRNA and double-stranded DNA oligonucleotides within the functionalized silica particles and provided evidence for the stability of the oligonucleotides inside the pores. Hence, our study demonstrates the potential of mesoporous silica particles as a means for alternative gene delivery in nanomedicine. Copyright © 2012 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim. |
| spellingShingle | Lebold, T Schlossbauer, A Schneider, K Schermelleh, L Leonhardt, H Bein, T Bräuchle, C Controlling the mobility of oligonucleotides in the nanochannels of mesoporous silica |
| title | Controlling the mobility of oligonucleotides in the nanochannels of mesoporous silica |
| title_full | Controlling the mobility of oligonucleotides in the nanochannels of mesoporous silica |
| title_fullStr | Controlling the mobility of oligonucleotides in the nanochannels of mesoporous silica |
| title_full_unstemmed | Controlling the mobility of oligonucleotides in the nanochannels of mesoporous silica |
| title_short | Controlling the mobility of oligonucleotides in the nanochannels of mesoporous silica |
| title_sort | controlling the mobility of oligonucleotides in the nanochannels of mesoporous silica |
| work_keys_str_mv | AT leboldt controllingthemobilityofoligonucleotidesinthenanochannelsofmesoporoussilica AT schlossbauera controllingthemobilityofoligonucleotidesinthenanochannelsofmesoporoussilica AT schneiderk controllingthemobilityofoligonucleotidesinthenanochannelsofmesoporoussilica AT schermellehl controllingthemobilityofoligonucleotidesinthenanochannelsofmesoporoussilica AT leonhardth controllingthemobilityofoligonucleotidesinthenanochannelsofmesoporoussilica AT beint controllingthemobilityofoligonucleotidesinthenanochannelsofmesoporoussilica AT brauchlec controllingthemobilityofoligonucleotidesinthenanochannelsofmesoporoussilica |