Quantitative 3D structured illumination microscopy of nuclear structures

3D structured illumination microscopy (3D-SIM) is the super-resolution technique of choice for multicolor volumetric imaging. Here we provide a validated sample preparation protocol for labeling nuclei of cultured mammalian cells, image acquisition and registration practices, and downstream image an...

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Những tác giả chính: Kraus, F, Miron, E, Demmerle, J, Chitiashvili, T, Budco, A, Alle, Q, Matsuda, A, Leonhardt, H, Schermelleh, L, Markaki, Y
Định dạng: Journal article
Ngôn ngữ:English
Được phát hành: Nature Publishing Group 2017