Quantitative 3D structured illumination microscopy of nuclear structures
3D structured illumination microscopy (3D-SIM) is the super-resolution technique of choice for multicolor volumetric imaging. Here we provide a validated sample preparation protocol for labeling nuclei of cultured mammalian cells, image acquisition and registration practices, and downstream image an...
Main Authors: | Kraus, F, Miron, E, Demmerle, J, Chitiashvili, T, Budco, A, Alle, Q, Matsuda, A, Leonhardt, H, Schermelleh, L, Markaki, Y |
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Format: | Journal article |
Language: | English |
Published: |
Nature Publishing Group
2017
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