Direct detection of membrane channels from gels using water-in-oil droplet bilayers.
We form planar lipid bilayers between an aqueous droplet and a hydrogel support immersed in a lipid-oil solution. By scanning the bilayer over the surface of an SDS-PAGE gel, we are able to directly detect membrane proteins from gels using single-channel recording. Using this technique, we are able...
Main Authors: | , , , |
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Format: | Journal article |
Language: | English |
Published: |
2007
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