Direct detection of membrane channels from gels using water-in-oil droplet bilayers.

We form planar lipid bilayers between an aqueous droplet and a hydrogel support immersed in a lipid-oil solution. By scanning the bilayer over the surface of an SDS-PAGE gel, we are able to directly detect membrane proteins from gels using single-channel recording. Using this technique, we are able...

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Bibliographic Details
Main Authors: Heron, A, Thompson, JR, Mason, A, Wallace, M
Format: Journal article
Language:English
Published: 2007