The improved procedure for the expression, purification and crystallization of mutants of cytochrome P450cam and the crystal structure of the F87L/Y96F/L244A/V247A mutant
The UV absorbance ratio (A392/A280) was developed to survey the purity of the P450cam mutant protein during the purification of the mutants protein. Thus the course of the purification became compact and the efficiency of the purification had been improved. β-Mercaptoethenol had been developed to ma...
| Main Authors: | , , , , , , |
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| פורמט: | Journal article |
| שפה: | Chinese |
| יצא לאור: |
2004
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