Protein-protein interaction site mapping using NMR-detected mutational scanning.

Protein-protein interaction site mapping using NMR-detected mutational scanning.

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We demonstrate a novel NMR method for the mapping of protein-protein interaction sites. In our approach protein-protein binding sites are mapped by competition binding experiments using indirect NMR reporter technology and Ala positional scanning. The methodology provides high sensitivity, ease of i...

书目详细资料
Main Authors:	Baminger, B, Ludwiczek, M, Kontaxis, G, Knapp, S, Konrat, R
格式:	Journal article
语言:	English
出版:	2007
主题:	genetics Reproducibility of Results Protein Interaction Mapping Models, Molecular TCF Transcription Factors beta Catenin Transcription Factor 7-Like 2 Protein Ligands methods Binding, Competitive metabolism Nuclear Magnetic Resonance, Biomolecular Mutation

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