Observation of the iron-sulfur cluster in Escherichia coli biotin synthase by nanoflow electrospray mass spectrometry.

Biotin synthase from Escherichia coli was analyzed by nanoflow electrospray ionization mass spectrometry. From solution conditions in which the protein is in its native state, a distribution of monomeric, dimeric, and tetrameric species was observed. The distribution of these species was sensitive t...

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Bibliographic Details
Main Authors: Hernández, H, Hewitson, K, Roach, P, Shaw, N, Baldwin, J, Robinson, C
Format: Journal article
Language:English
Published: 2001