Electrochemistry of single enzymes: fluctuations of catalase activities

Dynamic fluctuations of the catalytic ability of single catalase enzymes toward hydrogen peroxide decomposition are observed via the nanoimpact technique. The electrochemical signals of single enzymes show that the catalytic ability of single enzymes can temporarily be much higher than expected from...

詳細記述

書誌詳細
主要な著者: Lin, C, Sepunaru, L, Kätelhön, E, Compton, R
フォーマット: Journal article
言語:English
出版事項: American Chemical Society 2018
その他の書誌記述
要約:Dynamic fluctuations of the catalytic ability of single catalase enzymes toward hydrogen peroxide decomposition are observed via the nanoimpact technique. The electrochemical signals of single enzymes show that the catalytic ability of single enzymes can temporarily be much higher than expected from the classical, time-averaged Michaelis–Menten description. By combination of experimental data with a new theoretical model, we interpret the unusual enhancement of the single catalase signal and find that single catalases show large fluctuations of the catalytic ability.