An integrated taxonomy for monogenic inflammatory bowel disease
Background and aims Monogenic forms of inflammatory bowel disease (IBD) illustrate the essential roles of individual genes in pathways and networks safeguarding immune tolerance and gut homeostasis. Methods To build a taxonomy model we assessed 165 disorders. Genes were prioritized based on penetran...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Elsevier
2021
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| _version_ | 1797109761127219200 |
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| author | Bolton, C Smillie, CS Pandey, S Elmentaite, R Wei, G Argmann, C Aschenbrenner, D James, KR McGovern, DPB Macchi, M Cho, J Shouval, DS Kammermeier, J Koletzko, S Bagalopal, K Capitani, M Cavounidis, A Pires, E Weidinger, C McCullagh, J Arkwright, PD Haller, W Siegmund, B Peters, L Jostins, L Travis, SPL Anderson, CA Snapper, S Klein, C Schadt, E Zilbauer, M Xavier, R Teichmann, S Muise, AM Regev, A Uhlig, HH |
| author_facet | Bolton, C Smillie, CS Pandey, S Elmentaite, R Wei, G Argmann, C Aschenbrenner, D James, KR McGovern, DPB Macchi, M Cho, J Shouval, DS Kammermeier, J Koletzko, S Bagalopal, K Capitani, M Cavounidis, A Pires, E Weidinger, C McCullagh, J Arkwright, PD Haller, W Siegmund, B Peters, L Jostins, L Travis, SPL Anderson, CA Snapper, S Klein, C Schadt, E Zilbauer, M Xavier, R Teichmann, S Muise, AM Regev, A Uhlig, HH |
| author_sort | Bolton, C |
| collection | OXFORD |
| description | Background and aims
Monogenic forms of inflammatory bowel disease (IBD) illustrate the essential roles of individual genes in pathways and networks safeguarding immune tolerance and gut homeostasis.
Methods
To build a taxonomy model we assessed 165 disorders. Genes were prioritized based on penetrance of IBD and disease phenotypes were integrated with multi-omics datasets. Monogenic IBD genes were classified by: (1) overlapping syndromic features; (2) response to hematopoietic stem cell transplantation; (3) bulk RNA-seq of 32 tissues; (4) single-cell RNA-seq of >50 cell subsets from the intestine of healthy individuals and IBD patients (pediatric and adult), and (5) proteomes of 43 immune subsets. The model was validated by addition of newly identified monogenic IBD defects. As a proof-of-concept, we explore the intersection between immunometabolism and antimicrobial activity for a group of disorders (G6PC3/SLC37A4).
Results
Our quantitative integrated taxonomy defines the cellular landscape of monogenic IBD gene expression across 102 genes with high and moderate penetrance (81 in the model set and 21 genes in the validation set). We illustrate distinct cellular networks, highlight expression profiles across understudied cell types (e.g., CD8+ T cells, neutrophils, epithelial subsets and endothelial cells) and define genotype-phenotype associations (perianal disease and defective antimicrobial activity). We illustrate processes and pathways shared across cellular compartments and phenotypic groups and highlight cellular immunometabolism with mTOR activation as one of the converging pathways. There is an overlap of genes and enriched cell-specific expression between monogenic and polygenic IBD.
Conclusion
Our taxonomy integrates genetic, clinical and multi-omic data; providing a basis for genomic diagnostics and testable hypotheses for disease functions and treatment responses.
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| first_indexed | 2024-03-07T07:46:02Z |
| format | Journal article |
| id | oxford-uuid:d0962e30-ea06-4da2-8341-a0740fb49bb2 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:46:02Z |
| publishDate | 2021 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:d0962e30-ea06-4da2-8341-a0740fb49bb22023-05-26T16:58:26ZAn integrated taxonomy for monogenic inflammatory bowel diseaseJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d0962e30-ea06-4da2-8341-a0740fb49bb2EnglishSymplectic ElementsElsevier2021Bolton, CSmillie, CSPandey, SElmentaite, RWei, GArgmann, CAschenbrenner, DJames, KRMcGovern, DPBMacchi, MCho, JShouval, DSKammermeier, JKoletzko, SBagalopal, KCapitani, MCavounidis, APires, EWeidinger, CMcCullagh, JArkwright, PDHaller, WSiegmund, BPeters, LJostins, LTravis, SPLAnderson, CASnapper, SKlein, CSchadt, EZilbauer, MXavier, RTeichmann, SMuise, AMRegev, AUhlig, HHBackground and aims Monogenic forms of inflammatory bowel disease (IBD) illustrate the essential roles of individual genes in pathways and networks safeguarding immune tolerance and gut homeostasis. Methods To build a taxonomy model we assessed 165 disorders. Genes were prioritized based on penetrance of IBD and disease phenotypes were integrated with multi-omics datasets. Monogenic IBD genes were classified by: (1) overlapping syndromic features; (2) response to hematopoietic stem cell transplantation; (3) bulk RNA-seq of 32 tissues; (4) single-cell RNA-seq of >50 cell subsets from the intestine of healthy individuals and IBD patients (pediatric and adult), and (5) proteomes of 43 immune subsets. The model was validated by addition of newly identified monogenic IBD defects. As a proof-of-concept, we explore the intersection between immunometabolism and antimicrobial activity for a group of disorders (G6PC3/SLC37A4). Results Our quantitative integrated taxonomy defines the cellular landscape of monogenic IBD gene expression across 102 genes with high and moderate penetrance (81 in the model set and 21 genes in the validation set). We illustrate distinct cellular networks, highlight expression profiles across understudied cell types (e.g., CD8+ T cells, neutrophils, epithelial subsets and endothelial cells) and define genotype-phenotype associations (perianal disease and defective antimicrobial activity). We illustrate processes and pathways shared across cellular compartments and phenotypic groups and highlight cellular immunometabolism with mTOR activation as one of the converging pathways. There is an overlap of genes and enriched cell-specific expression between monogenic and polygenic IBD. Conclusion Our taxonomy integrates genetic, clinical and multi-omic data; providing a basis for genomic diagnostics and testable hypotheses for disease functions and treatment responses. |
| spellingShingle | Bolton, C Smillie, CS Pandey, S Elmentaite, R Wei, G Argmann, C Aschenbrenner, D James, KR McGovern, DPB Macchi, M Cho, J Shouval, DS Kammermeier, J Koletzko, S Bagalopal, K Capitani, M Cavounidis, A Pires, E Weidinger, C McCullagh, J Arkwright, PD Haller, W Siegmund, B Peters, L Jostins, L Travis, SPL Anderson, CA Snapper, S Klein, C Schadt, E Zilbauer, M Xavier, R Teichmann, S Muise, AM Regev, A Uhlig, HH An integrated taxonomy for monogenic inflammatory bowel disease |
| title | An integrated taxonomy for monogenic inflammatory bowel disease |
| title_full | An integrated taxonomy for monogenic inflammatory bowel disease |
| title_fullStr | An integrated taxonomy for monogenic inflammatory bowel disease |
| title_full_unstemmed | An integrated taxonomy for monogenic inflammatory bowel disease |
| title_short | An integrated taxonomy for monogenic inflammatory bowel disease |
| title_sort | integrated taxonomy for monogenic inflammatory bowel disease |
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